Hydrogen Deuterium Exchange Mass Spectrometry Services
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Binding Site Characterization
HDX MS is a FDA-approved methodology to validate the structural integrity of biosimilars.
Additionally, HDX MS is use to as a tool for monitoring the integrity of the production process, such as batch-to-batch reproducibility and formulation stability.
HDX MS is routinely utilized to identify the binding site of small molecules to proteins, as well as for the characterization of binding interfaces between protein/protein and protein/peptide complexes.
The analysis can performed in samples containing almost any kind of biological buffers (e.g TRIS, BIS-TRIS, HEPES), and using 0.2 to 1 mg of protein.
HDX MS can be used for ligand screening by monitoring areas related to the binding site. The LC-MS and data processing are tailored to interrogate a library of compounds to a target protein.
The analysis can performed in samples containing almost any kind of biological buffers (e.g TRIS, BIS-TRIS, HEPES).